服務(wù)項(xiàng)目

(Service Items)

周期（周）

Time(weeks)

免疫庫

immunized   library

天然庫

naive library

雜交瘤制備篩選

Hybridoma   Screening

多肽設(shè)計(jì)與合成

Peptide design   & synthesis

多肽設(shè)計(jì)

Peptide design

~2

可選，免費(fèi)

多肽合成

Peptide   synthesis

√

√

小分子或多肽偶聯(lián)

conjugation

√

√

蛋白制備

Proteins   preparation

cDNA合成

cDNA synthesis

6~8

√

另議，時間與價格均不包含在以下信息中

載體構(gòu)建

Vector   construction

√

√

蛋白表達(dá)與純化

Protein   expression & purification

√

√

免疫階段

Immunization

免疫動物

Immunized   animals

~8

√

√

ELISA檢測

Elisa detection

√

√

細(xì)胞融合與篩選

Fusion &   Screening

1.5

√

亞克隆階段

Subcloning

1

√

腹水生產(chǎn)（2只大鼠）

Ascites   production (Injection on 2 mice)

1

√

cDNA制備

cDNA preparation

逆轉(zhuǎn)錄制備cDNA文庫

prepare cDNA   library by RT-PCR

~1

√

cDNA 文庫質(zhì)檢

cDNA library QC   check

√

構(gòu)建Fab抗體庫

Fab library   construction

VH與VL基因獲得

obtained VH   and VL genes

10~15

√

構(gòu)建初級抗體庫

primary antibody   library construction

√

構(gòu)建Fab抗體庫

Fab library   construction

√

噬菌體篩選

phage screening

噬菌體顆粒包裝

phage particles   package

10~15

√

√

磁珠親和篩選

magnetic beads   affinity screening

√

√

陽性克隆回收基因測序

Recover positive   clones and DNA sequencing

√

√

抗體表達(dá)

Antibody   expression

特異性雙鏈基因合成與Fc基因橋接

synthetic of   target phage gene and Fc gene

~5

√

√

構(gòu)建真核表達(dá)載體

Eukaryotic   system expression plasmids construction

√

√

真核表達(dá)

Eukaryotic   system expression

√

√

穩(wěn)轉(zhuǎn)細(xì)胞系構(gòu)建

Stably   transfected cell line construction

純化與檢測

Purification and   detection

標(biāo)簽純化

Tag affinity   purification

~1

√

√

親和純化

Affinity   purification

√

特異性親和純化

Antigen specific   affinity purification

√

√

總周期（月）

Total lead   time(months)

7~9